Novel spectrophotometric determination of Valacyclovir and Cefotaxime using 1, 2-napthaquinone-4-sulfonic acid sodium in bulk and pharmaceutical dosage form
نویسندگان
چکیده
A new, simple and sensitive spectrophotometric method for the determination of valacyclovir and cefotaxime has been developed. The method is based on the condensation of valacyclovir and cefotaxime with 1, 2napthaquinone-4sulfonic acid sodium (NQS) in alkaline media to yield orange colored products respectively. Valacyclovir and cefotaxime showed maximum absorbance at 495nm and 475nm with linearity was observed in the concentration range of 20120 μg/ml and 20-140 μg/ml respectively. The relative standard deviations of 0.363% for valacyclovir and 0.66% for cefotaxime were obtained. The recoveries of valacyclovir and cefotaxime injections are in the range 96.01±0.52 and 98.12±0.96 respectively. The proposed method is simple, rapid, precise and convenient for the assay of valacyclovir and cefotaxime in commercial injection preparations.
منابع مشابه
Spectrophotometric Determination of Valacyclovir in Pharmaceutical Formulations
A simple spectrophotometric method has been developed for the estimation of valacyclovir in pharmaceutical preparations. The method is based on the reaction of the valacyclovir with methanolic solution of para dimethyl amino benzaldehyde (PDAB) in acidic condition producing Schiff base having absorption maximum at 400 nm. Beer’s law is obeyed in the concentration of 50-250 μg/mL of valacyclovir...
متن کاملSimultaneous RP-HPLC and UV Spectroscopic Method Development and Validation for Estimation of Ibandronate Sodium in Bulk and Pharmaceutical Dosage Form
The present study describes a simple, accurate, precise and cost effective UV-Vis Spectroscopic and RP-HPLC method for the estimation of Ibandronate sodium (IBN). The determination of Ibandronate sodium (IBN) was performed by both UV and RP-HPLC method using 215 nm as the determination wavelength. The drug was dissolved in NaOH solution (0.1N NaOH) for estimation in UV and in distilled water fo...
متن کاملDevelopment and Validation of a Simple UV Spectrophotometric Method for the Determination of Cefotaxime Sodium in Bulk And Pharmaceutical Formulation
The present study was undertaken to develop and validate a simple, accurate, precise, reproducible and cost effective UV-Visible spectrophotometric method for the estimation of cefotaxime sodium in bulk and pharmaceutical formulation. The solvent used throughout the experiment was methanol and water. Absorption maximum (λmax) of the drug was found to be 260 nm. The quantitative determination of...
متن کاملTitrimetric and Spectrophotometric Methods for the Determination of β-blockers in Pharmaceutical Dosage Form
Three new methods using titrimetric and spectrophotometry are described for the determination of Propranolol Hydrochloride (PPH) and Atenolol (AN) with potassium bromate as the oxidimetric reagent and acid dyes, Methyl orange and Indigo carmine. In direct titrimetric (method A), the drug is titrated directly with bromate in acid medium and in the presence of excess of bromide using methyl orang...
متن کاملSpectrophotometric Assay of Valacyclovir in Pharmaceutical Dosage Forms
A simple and reproducible spectrophotometric method has been developed for the determination of valacyclovir in bulk and pharmaceutical dosage forms. The proposed method was based on the formation of chloroform extractable complex of valacyclovir with wool fast blue. The absorbance of the extractable ion pair complex is measured at the wavelength of maximum absorbance 585 nm against the reagent...
متن کامل